@article {2615,
	title = {Microbial decomposition of marine dissolved organic matter in cool oceanic crust},
	journal = {Nature Geoscience},
	volume = {11},
	year = {2018},
	month = {Jan-05-2018},
	pages = {334 - 339},
	abstract = {Marine dissolved organic carbon (DOC) is one of the largest active reservoirs of reduced carbon on Earth. In the deep ocean, DOC has been described as biologically recalcitrant and has a radiocarbon age of 4,000 to 6,000 years, which far exceeds the timescale of ocean overturning. However, abiotic removal mechanisms cannot account for the full magnitude of deep-ocean DOC loss. Deep-ocean water circulates at low temperatures through volcanic crust on ridge flanks, but little is known about the associated biogeochemical processes and carbon cycling.
Here we present analyses of DOC in fluids from two borehole observatories installed in crustal rocks west of the Mid-Atlantic Ridge, and show that deep-ocean DOC is removed from these cool circulating fluids. The removal mechanism is isotopically selective and causes a shift in specific features of molecular composition, consistent with microbe-mediated oxidation. We suggest organic molecules with an average radiocarbon age of 3,200 years are bioavailable to crustal microbes, and that this removal mechanism may account for at least 5\% of the global loss of DOC in the deep ocean. Cool crustal circulation probably contributes to maintaining the deep ocean as a reservoir of {\textquoteright}aged{\textquoteright} and refractory DOC by discharging the surviving organic carbon constituents that are molecularly degraded and depleted in C-14 and C-13 into the deep ocean.},
	keywords = {carbon, community, deep-ocean, DISTINCT, extraction, fluid-flow, HEAT, MID-ATLANTIC RIDGE, SEAWATER, WESTERN FLANK},
	issn = {1752-0894},
	doi = {10.1038/s41561-018-0109-5},
	url = {http://www.nature.com/articles/s41561-018-0109-5http://www.nature.com/articles/s41561-018-0109-5.pdfhttp://www.nature.com/articles/s41561-018-0109-5http://www.nature.com/articles/s41561-018-0109-5.pdf},
	author = {Shah Walter, Sunita R. and Jaekel, Ulrike and Osterholz, Helena and Fisher, Andrew T. and Huber, Julie A. and Pearson, Ann and Dittmar, Thorsten and Girguis, Peter R.}
}
@conference {1692,
	title = {Microscale AMS (super <sup>14</sup>) C measurement at NOSAMS},
	volume = {40},
	year = {1998},
	note = {id: 2237; Source type: conferencepapers\&proceedings; Object type: Article; Object type: Conference Paper; Copyright: GeoRef, Copyright 2007, American Geological Institute.; CSAUnique: 1999-044438; AccNum: 1999-044438; ISSN: 0033-8222; CODEN: RACAAT},
	month = {1998},
	pages = {61-75},
	publisher = {University of Arizona, Department of Geosciences, Tucson, AZ, United States (USA)},
	organization = {University of Arizona, Department of Geosciences, Tucson, AZ, United States (USA)},
	address = {United States (USA)},
	abstract = {Techniques for making precise and accurate radiocarbon accelerator mass spectrometry (AMS) measurements on samples containing less than a few hundred micrograms of carbon are being developed at the NOSAMS facility. A detailed examination of all aspects of the sample preparation and data analysis process shows encouraging results. Small quantities of CO (sub 2) are reduced to graphite over cobalt catalyst at an optimal temperature of 605 degrees C. Measured (super 14) C/ (super 12) C ratios of the resulting targets are affected by machine-induced isotopic fractionation, which appears directly related to the decrease in ion current generated by the smaller sample sizes. It is possible to compensate effectively for this fractionation by measuring samples relative to small standards of identical size. Examination of the various potential sources of background (super 14) C contamination indicates that the sample combustion process is the largest contributor, adding ca. 1 mu g of carbon with a less-than-modern (super 14) C concentration.},
	keywords = {03, absolute age, accelerator mass spectroscopy, accuracy, C-14, C-14/C-12, carbon, Carbon dioxide, combustion, data processing, Geochronology, graphite, isotope fractionation, isotopes, mass spectroscopy, mathematical methods, methods, native elements, radioactive isotopes, sample preparation, size, spectroscopy, Stable isotopes},
	doi = {10.1017/S0033822200017902},
	url = {http://hdl.handle.net/10150/653650},
	author = {Pearson, Ann and McNichol, Ann P. and Schneider, Robert J. and von Reden, Karl F. and Zheng, Yan}
}
@conference {1800,
	title = {(super 14) C AMS measurements of},
	volume = {40},
	year = {1998},
	note = {id: 2239; Source type: conferencepapers\&proceedings; Object type: Article; Object type: Conference Paper; Copyright: GeoRef, Copyright 2007, American Geological Institute.; CSAUnique: 1999-044459; AccNum: 1999-044459; ISSN: 0033-8222; CODEN: RACAAT},
	month = {1998},
	pages = {247-253},
	publisher = {University of Arizona, Department of Geosciences, Tucson, AZ, United States (USA)},
	organization = {University of Arizona, Department of Geosciences, Tucson, AZ, United States (USA)},
	address = {United States (USA)},
	abstract = {The NOSAMS facility at Woods Hole Oceanographic Institution has started to develop and apply techniques for measuring very small samples on a standard Tandetron accelerator mass spectrometry (AMS) system with high-current hemispherical Cs sputter ion sources. Over the past year, results on samples ranging from 7 to 160 mu g C showed both the feasibility of such analyses and the present limitations on reducing the size of solid carbon samples. One of the main factors affecting the AMS results is the dependence of a number of the beam optics parameters on the extracted ion beam current. The extracted currents range from 0.5 to 10 mu A of (super 12) C (super -) for the sample sizes given above. We here discuss the setup of the AMS system and methods for reliable small-sample measurements and give the AMS-related limits to sample size and the measurement uncertainties.},
	keywords = {03, absolute age, accelerator mass spectroscopy, C-14, carbon, dilution, Geochronology, ions, isotopes, mass spectroscopy, precision, radioactive isotopes, sample preparation, spectroscopy, volume},
	author = {von Reden, Karl F. and McNichol, Ann P. and Pearson, Ann and Schneider, Robert J.}
}
