@article {1003, title = {Algal and archaeal polyisoprenoids in a recent marine sediment: Molecular isotopic evidence for anaerobic oxidation of methane}, journal = {Geochemistry Geophysics Geosystems}, volume = {2}, year = {2001}, note = {458jvTimes Cited:51Cited References Count:79}, month = {Jan 17}, abstract = {Analyses of C-13 contents of individual organic molecules in a marine sediment show that crocetane, 2,6,11,15-tetramethylhexadecane, an isomer of phytane, is produced by microorganisms that use methane as their main source of carbon. The sediments lie at a water depth of 68 m in the Kattegat, the strait between Denmark and Sweden. Crocetane appears first 185 cm below the sediment-water interface, in the zone marking the transition from sulfate reduction to methanogenesis. Its delta C-13 value is -90 +/- 10 parts per thousand versus Vienna Pee Dee Belemnite (VPDB). Its structure, which includes four isoprene units arranged symmetrically around a tail-to-tail linkage, suggests that it is produced by a member of the archaea. Growing at the intersection of the diffusion gradients for sulfate and methane in sedimentary pore waters, the source organism apparently function as a methane-consuming member of the microbial consortium responsible for the anaerobic oxidation of methane [Hoehler et al., 1994], in which, as first demonstrated quantitatively in these sediments [Iversen and Jorgensen, 1985], electrons are transferred from methane to sulfate. The presence of archaeal biomass throughout the sediment section is indicated by significant concentrations of 2,6,10,15,19-pentamethylicosane (PMI) and of ether-bound phytane and biphytane. The PMI reaches a minimum delta value of -47 parts per thousand well below the transition zone. Its isotopic depletion could reflect either methanogenic or methanotrophic sources. The ether-bound lipids are isotopically uniform throughout the section and are presumed to derive from archaea that utilize a carbon source unaffected by the oxidation of methane.}, issn = {1525-2027}, doi = {10.1029/2000GC000112}, author = {Bian, L. Q. and Hinrichs, K. U. and Xie, T. M. and Brassell, S. C. and Iversen, H. and Fossing, H. and Jorgensen, B. B. and Hayes, J. M.} } @article {692, title = {Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments}, journal = {Appl Environ Microbiol}, volume = {67}, year = {2001}, note = {Orphan, V JHinrichs, K UUssler, W 3rdPaull, C KTaylor, L TSylva, S PHayes, J MDelong, E FengComparative StudyResearch Support, Non-U.S. Gov{\textquoteright}tResearch Support, U.S. Gov{\textquoteright}t, Non-P.H.S.2001/04/03 10:00Appl Environ Microbiol. 2001 Apr;67(4):1922-34.}, month = {Apr}, pages = {1922-34}, abstract = {The oxidation of methane in anoxic marine sediments is thought to be mediated by a consortium of methane-consuming archaea and sulfate-reducing bacteria. In this study, we compared results of rRNA gene (rDNA) surveys and lipid analyses of archaea and bacteria associated with methane seep sediments from several different sites on the Californian continental margin. Two distinct archaeal lineages (ANME-1 and ANME-2), peripherally related to the order Methanosarcinales, were consistently associated with methane seep marine sediments. The same sediments contained abundant (13)C-depleted archaeal lipids, indicating that one or both of these archaeal groups are members of anaerobic methane-oxidizing consortia. (13)C-depleted lipids and the signature 16S rDNAs for these archaeal groups were absent in nearby control sediments. Concurrent surveys of bacterial rDNAs revealed a predominance of delta-proteobacteria, in particular, close relatives of Desulfosarcina variabilis. Biomarker analyses of the same sediments showed bacterial fatty acids with strong (13)C depletion that are likely products of these sulfate-reducing bacteria. Consistent with these observations, whole-cell fluorescent in situ hybridization revealed aggregations of ANME-2 archaea and sulfate-reducing Desulfosarcina and Desulfococcus species. Additionally, the presence of abundant (13)C-depleted ether lipids, presumed to be of bacterial origin but unrelated to ether lipids of members of the order Desulfosarcinales, suggests the participation of additional bacterial groups in the methane-oxidizing process. Although the Desulfosarcinales and ANME-2 consortia appear to participate in the anaerobic oxidation of methane in marine sediments, our data suggest that other bacteria and archaea are also involved in methane oxidation in these environments.}, issn = {0099-2240 (Linking)}, doi = {10.1128/AEM.67.4.1922-1934.2001}, author = {Orphan, V. J. and Hinrichs, K. U. and Ussler, W. and Paull, C. K. and Taylor, L. T. and Sylva, S. P. and Hayes, J. M. and DeLong, E. F.} } @article {814, title = {Molecular and isotopic analysis of anaerobic methane-oxidizing communities in marine sediments}, journal = {Organic Geochemistry}, volume = {31}, year = {2000}, note = {384quTimes Cited:204Cited References Count:49}, pages = {1685-1701}, abstract = {Convergent lines of molecular, carbon-isotopic, and phylogenetic evidence have previously indicated (Hinrichs, K.U., Hayes, J.M., Sylva, S.P., Brewer. P.G.. DeLong, E.F., 1999. Methane-consuming archaebacteria in marine sediments. Nature 398, 802-805.) that archaea are involved in the anaerobic oxidation of methane in sediments from the Eel River Basin. offshore northern California. Now, further studies of those same sediments and of sediments from a methane seep in the Santa Barbara Basin have confirmed and extended those results. Mass spectrometric and chromatographic analyses of an authentic standard of sn-2-hydroxyarchaeol (hydroxylated at C-3 in the sn-2 phytanyl moiety) have confirmed our previous, tentative identification of this compound but shown that the previously examined product was the mono-TMS, rather than di-TMS, derivative. Further analyses of C-13-depleted lipids, appreciably more abundant in samples from the Santa Barbara Basin, have shown that the archaeal lipids are accompanied by two sets of products that are only slightly less depleted in C-13. These are additional glycerol ethers and fatty acids. The alkyl substituents in the ethers (mostly monoethers, with some diethers) are non-isoprenoidal. The carbon-number distributions and isotopic compositions of the alkyl substituents and of the fatty acids are similar, suggesting strongly that they are produced by the same organisms. Their structures, n-alkyl and methyl-branched n-alkyl, require a bacterial rather than archaeal source. The non-isoprenoidal glycerol ethers are novel constituents in marine sediments but have been previously reported in thermophilic, sulfate- and nitrate-reducing organisms which lie near the base of the rRNA-based phylogenetic tree. Based on previous observations that the anaerobic oxidation of methane involves a net transfer of electrons from methane to sulfate, it appears likely that the non-archaeal, C-13-depleted lipids are products of one or more previously unknown sulfate-reducing bacteria which grow syntrophically with the methane-utilizing archaea. Their products account for 50\% of the fatty acids in the sample from the Santa Barbara Basin. At all methane-seep sites examined, the preservation of aquatic products is apparently enhanced because the methane-oxidizing consortium utilizes much of the sulfate that would otherwise be available for remineralization of materials from the water column. Crown Copyright (C) 2000 Published by Elsevier Science Ltd. All rights reserved.}, issn = {0146-6380}, doi = {10.1016/S0146-6380(00)00106-6}, author = {Hinrichs, K. U. and Summons, R. E. and Orphan, V. and Sylva, S. P. and Hayes, J. M.} } @article {635, title = {Methane-consuming archaebacteria in marine sediments}, journal = {Nature}, volume = {398}, year = {1999}, note = {Hinrichs, K UHayes, J MSylva, S PBrewer, P GDeLong, E FengResearch Support, Non-U.S. Gov{\textquoteright}tResearch Support, U.S. Gov{\textquoteright}t, Non-P.H.S.England1999/05/11 02:03Nature. 1999 Apr 29;398(6730):802-5.}, month = {Apr 29}, pages = {802-5}, abstract = {Large amounts of methane are produced in marine sediments but are then consumed before contacting aerobic waters or the atmosphere. Although no organism that can consume methane anaerobically has ever been isolated, biogeochemical evidence indicates that the overall process involves a transfer of electrons from methane to sulphate and is probably mediated by several organisms, including a methanogen (operating in reverse) and a sulphate-reducer (using an unknown intermediate substrate). Here we describe studies of sediments related to a decomposing methane hydrate. These provide strong evidence that methane is being consumed by archaebacteria that are phylogenetically distinct from known methanogens. Specifically, lipid biomarkers that are commonly characteristic of archaea are so strongly depleted in carbon-13 that methane must be the carbon source, rather than the metabolic product, for the organisms that have produced them. Parallel gene surveys of small-subunit ribosomal RNA (16S rRNA) indicate the predominance of a new archael group which is peripherally related to the methanogenic orders Methanomicrobiales and Methanosarcinales.}, issn = {0028-0836 (Linking)}, doi = {10.1038/19751}, author = {Hinrichs, K. U. and Hayes, J. M. and Sylva, S. P. and Brewer, P. G. and DeLong, E. F.} }